Isolation of an anaerobic bacterium which reductively dechlorinates tetrachloroethene and trichloroethene

✍ Scribed by Alex Wild; René Hermann; Thomas Leisinger

• Book ID: 104626199
• Publisher: Springer Netherlands
• Year: 1997
• Tongue: English
• Weight: 605 KB
• Volume: 7
• Category: Article
• ISSN: 0923-9820
• DOI: 10.1007/bf00115297

✦ Synopsis

Strain TEA, a strictly anaerobic, motile rod with one to four lateral flagella and a crystalline surface layer was isolated from a mixed culture that completely reduces chlorinated ethenes to ethene. The organism coupled reductive dehalogenation of tetrachloroethene or trichloroethene to cis-1,2-dichloroethene to growth, using molecular hydrogen as the electron donor. It was unable to grow fermentatively or in the presence of tri- or tetrachloroethene with glucose, pyruvate, lactate, acetate or formate. The 16S rDNA sequence of strain TEA was 99.7% identical to that of Dehalobacter restrictus. The two organisms thus are representatives of the same species or the same genus within the Bacillus/Clostridium subphylum of the gram-positive bacteria.