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Isolation of activated adult schwann cells and a spontaneously immortal schwann cell clone

✍ Scribed by Dr. L. M. Bolin; T. P. Iismaa; E. M. Shooter

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 868 KB
Volume: 33
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490330206

No coin nor oath required. For personal study only.

✦ Synopsis

Successful mammalian peripheral nerve regeneration is dependent on activated Schwann cells. Schwann cells facilitate neuronal regrowth through the production of tropic cell membrane molecules, neurotrophins, and extracellular matrix components. To better understand Schwann cell function in the regenerating nerve, we have designed a method of isolating proliferating adult Schwann cells from the injured rat sciatic nerve. Relying on the mitotic signal that is present after a crush injury, we can obtain sufficient numbers of dividing Schwann cells within one week of initial culture. A spontaneously immortal Schwann cell clone (ISC) was observed in and isolated from one of these primary cultures. These cells were transformed at a time of maximal Schwann cell activation in response to injury. Both the primary Schwann cells and the iSC have been characterized as Schwann cells by morphology, immunohistochemistry and gene expression.

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