Isolation of a stable apical segment of the guinea pig sperm acrosome
β Scribed by Stojanoff, A. ;Bourne, H. ;Andrews, A. G. ;Hyne, R. V.
- Book ID
- 111663262
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1987
- Weight
- 812 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0148-7280
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In adapting several methods of membrane isolation we established a successful way to purify apical and basolateral membranes of guinea pig colon in a parallel procedure. The conventional purification control by marker enzymes was applied. In addition, luminal membrane proteins were stained with Texa