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Isolation of a Serratia marcescens mutant which is an efficient recipient for the E. coli episome

โœ Scribed by Takata, Renkichi ;Aoyagi, Michiko


Book ID
104724818
Publisher
Springer
Year
1984
Tongue
English
Weight
244 KB
Volume
197
Category
Article
ISSN
0026-8925

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โœฆ Synopsis


A Serratia marcescens mutant, which is an efficient recipient for the Escherichia coli episome (F' plasmid), was isolated after mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine (NTG). Episomes could be maintained in the mutant cell under conditions selective for a gene on the plasmid. This S. marcescens mutant could also be transformed with pBR 322 DNA at a frequency higher than that of the parental strain.


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