Isolation of a napin-like polypeptide with potent translation-inhibitory activity from chinese cabbage (Brassica parachinensis cv green-stalked) seeds
✍ Scribed by Patrick H. K. Ngai; Dr T. B. Ng
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 110 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1075-2617
- DOI
- 10.1002/psc.460
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✦ Synopsis
Abstract
A heterodimeric napin‐like polypeptide was isolated from Brassica parachinensis seeds with a procedure involving ion exchange chromatography on DEAE‐cellulose, affinity chromatography on Affi‐gel blue gel, FPLC‐ion exchange chromatography on Mono S and FPLC‐gel filtration on Superdex 75. The N‐terminal sequence of the 5 kDa subunit of the polypeptide (PAGPFRIPKKRKKEE) showed high homology with other 2S storage proteins like napins and albumins. The polypeptide potently inhibited translation in a cell free system with an IC~50~ of 6.2 nM. The translation‐inhibiting activity of the polypeptide was relatively stable in the pH range 6–11 and in the temperature range 10–50°C. Copyright © 2003 European Peptide Society and John Wiley & Sons, Ltd.