Isolation of a murine liver-specific alloantigen, F antigen, and examination of its immunogenic properties by radioimmunoassay
✍ Scribed by D. P. Lane; D. M. Silver
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 745 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Abstract
The autoantibody response induced in mice by alloimmunization with liver‐specific F antigen is an example of the circumvention of humoral self‐tolerance. The F molecule has been isolated and shown to be a protein with a mol. wt. of 40 000 Daltons which migrated as a β‐globulin. The purified antigen retains both the immunogenic and antibody‐combining properties of crude liver homogenate. Successful iodination of the protein allowed the establishment of a radioimmunoassay which demonstrated high titers of antibodies in responder strains. At the same time it was clearly shown that no antibody could be detected, either as a result of syngeneic immunization or as a result of alloimmunization in nonresponder strains. The characteristics of the F antigen immune response distinguish it from that induced by other proteins.