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Isolation of a DNA fraction highly enriched in transfer RNA genes from Xenopus laevis

✍ Scribed by Francesca Andronico; Elena Beccari


Book ID
115745982
Publisher
Elsevier Science
Year
1975
Weight
549 KB
Volume
395
Category
Article
ISSN
0005-2787

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A detergent-citric acid technique for is
✍ Leo Miller πŸ“‚ Article πŸ“… 1979 πŸ› Elsevier Science 🌐 English βš– 722 KB

A simple technique is described for fractionating cells ofxenopus laevis using Triton X-100 and citric acid. Cells are treated for 1 min with a hypotonic buffer containing 0.5% Triton X-100 and RNAase inhibitors. The cells are then disrupted by adding citric acid to a final concentration of 1% for 2