A detergent-citric acid technique for is
β
Leo Miller
π
Article
π
1979
π
Elsevier Science
π
English
β 722 KB
A simple technique is described for fractionating cells ofxenopus laevis using Triton X-100 and citric acid. Cells are treated for 1 min with a hypotonic buffer containing 0.5% Triton X-100 and RNAase inhibitors. The cells are then disrupted by adding citric acid to a final concentration of 1% for 2