Specialized transducing 2 phages have been isolated which carry the regions of the Escherichia coli chromosome containing the gene or gene-clusters for ribosomal proteins (r-proteins) S 1, $6-S 18-L9, S 16-L 19, and L28-L33. To investigate whether these phages also carry the genes for r-proteins $9,
Isolation of a Complex between the P Protein of Phage λ and the dnaB Protein of Escherichia coli
✍ Scribed by Albrecht KLEIN; Erich LANKA; Heinz SCHUSTER
- Book ID
- 115118687
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 605 KB
- Volume
- 105
- Category
- Article
- ISSN
- 1432-1327
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Fragments of the E. coli chromosome that carry the dnaB groPB534 or groPB612 alleles have been cloned into a cosmid vector. The resulting recombinant plasmids contained the genes uvrA, groP (B534 or B612), and lexA. Further subcloning into high copy number plasmids, during which the uvrA and lexA ge
The interaction between the dnaB protein of E. coli and the gene P product of lambda bacteriophage was investigated by measuring the cleavage of closed circular phage DNA after infection of two temperature sensitive dnaB mutants, JG28 and To534 groP- B. Cleavage of superhelical DNA from a lambda pi