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Isolation of a cDNA fragment coding for Chlamydomonas reinhardtii ferredoxin and expression of the recombinant protein in Escherichia coli

✍ Scribed by W.John Rogers; Michael Hodges; Paulette Decottignies; Jean-Marie Schmitter; Pierre Gadal; Jean-Pierre Jacquot


Book ID
115927010
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
677 KB
Volume
310
Category
Article
ISSN
0014-5793

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✍ Little, John W. πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 1002 KB

The E. coli recA gene was cloned from the phage lambda precA into the vector pBR313. A plasmid, pJL3, was also isolated by cloning a portion of the recA gene into the vector pBR322. pJL3 coded for a fragment of the recA protein 34 Kd (kilodaltons) in size (compared to 40 Kd for the intact protein).