๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Isolation of 8-methoxypsoralen accessible DNA domains from chromatin of intact cells

โœ Scribed by Georg A. Mathis; Felix R. Althaus

Book ID
104635711
Publisher
Springer
Year
1990
Tongue
English
Weight
1015 KB
Volume
6
Category
Article
ISSN
0742-2091

No coin nor oath required. For personal study only.

โœฆ Synopsis

The chromatin organization of living mammalian cells was probed using 8-methoxypsoralen (MOP). In intact cells, MOP intercalates into DNA domains which are also preferentially accessible to micrococcal nuclease. After UV365 nm irradiation of MOP-treated cells, this chemical forms bifunctional adducts crosslinking the two strands of DNA. Following extraction of cellular DNA, heat denaturation and renaturation at low temperature, the fraction of crosslinked DNA is obtained following enzymatic hydrolysis of unhybridized, non-crosslinked DNA by nuclease S1 treatment. An application of this procedure in the isolation of 8-methoxypsoralenaccessible DNA domains during DNA excision repair is shown.

๐Ÿ“œ SIMILAR VOLUMES

Effect of nickel(II) on DNA-protein bind
Effect of nickel(II) on DNA-protein binding, thymidine incorporation, and sedimentation pattern of chromatin fractions from intact mammalian cells
โœ Patierno, Steven R. ;Sugiyama, Masayasu ;Costa, Max ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 642 KB

Nuclear uptake and chromatin binding of nickel(II) was investigated in Chinese hamster ovary (CHO) cells. The cytoplasmic:nuclear ratio of nickel immediately following treatment was 5:1, but by 24 and 48 hours this ratio decreased to 4:1 and 2:1, respectively, indicating that nickel is retained long