## Abstract The frontal cortex showed a marked elevation of incorporation of HโLโfucose into glycoproteins of the crude synaptosomal fraction at 9 days of age. The effect was observed at both 8 and 24 hr after intraperitoneal administration of radioactive fucose. The elevation was absent in the 1,
Isolation from bovine brain of a fraction containing capillaries and a fraction containing membrane fragments of the choroid plexus
โ Scribed by Sessa, Grazia ;Orlowski, Marian ;Green, Jack Peter
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 740 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0022-3034
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A simple technique is described for fractionating cells ofxenopus laevis using Triton X-100 and citric acid. Cells are treated for 1 min with a hypotonic buffer containing 0.5% Triton X-100 and RNAase inhibitors. The cells are then disrupted by adding citric acid to a final concentration of 1% for 2
A convenient, large-scale method for the isolation of membrane vesicles permeable to specific inorganic ions has been developed. The general principle of this method involves the exchange of Na+ within the vesicles for external Cs+. Vesicles in which this exchange rapidly occurs can be separated on