๐”– Bobbio Scriptorium
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Isolation, culture and morphogenesis from wheat protoplasts, and study of expression of DNA constructs by direct gene transfer

โœ Scribed by B. T. Lee; K. Murdoch; J. Topping; M. T. J. Both; Q. S. Wu; A. Karp; S. Steele; C. Symonds; M. Kreis; M. G. K. Jones

Publisher
Springer
Year
1988
Tongue
English
Weight
317 KB
Volume
12
Category
Article
ISSN
0167-6857

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A rapid method to study heterogeneous ge
A rapid method to study heterogeneous gene expression in liver by direct assay of messenger RNA from periportal and perivenous cell lysates
โœ Juhani Saarinen; Ritva Saarelainen; Kai O. Lindros ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 435 KB

To study zonation of liver gene expression, we obtained periportal or perivenous rat liver cell lysates virtually devoid of nuclear material by site-directed digitonin infusion in situ. Total RNA was isolated, messenger RNAs were reverse transcribed and complementary DNAs were assayed after polymera