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Isolation, characterization, and development of WRKY genes as useful genetic markers inTheobroma cacao

✍ Scribed by James W. Borrone; David N. Kuhn; Raymond J. Schnell


Book ID
105813477
Publisher
Springer
Year
2004
Tongue
English
Weight
363 KB
Volume
109
Category
Article
ISSN
0040-5752

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A system is described for gene disruption and replacement in Schizosaccharomyces pombe based on the homologous selectable marker, ura4, the structural gene for orotidine-5'-phosphate decarboxylase. The presence of a single copy of the wild-type gene can rescue a ura4 auxotrophic mutant. Furthermore,