Isolation and Structural Elucidation of Steroid Oligoglycosides from the Starfish Asteriasrubens by Means of Direct Online LC-NMR-MS Hyphenation and One- and Two-Dimensional NMR Investigations

✍ Scribed by Martin Sandvoss; Lam Huong Pham; Karsten Levsen; Alfred Preiss; Clemens Mügge; Gerold Wünsch

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 407 KB
• Volume: 2000
• Category: Article
• ISSN: 1434-193X
• DOI: 10.1002/1099-0690(200004)2000:7<1253::aid-ejoc1253>3.0.co;2-g

✦ Synopsis

LC-NMR-MS hyphenation has been applied in the screening of an asterosaponin subfraction obtained from the starfish Asterias rubens for unknown compounds. The suitability of this technique for characterizing compounds in the molecular weight range of 1200 to 1400 a.m.u. has been demonstrated. The additional information concerning the number of exchangeable protons provided by the LC-NMR-MS experiment proved to be particularly valuable for the structural elu-