Isolation and some properties of beta-hemolysin produced byNocardia asteroides

✍ Scribed by A. C. Emeruwa

• Publisher: Springer Netherlands
• Year: 1986
• Tongue: English
• Weight: 851 KB
• Volume: 95
• Category: Article
• ISSN: 0301-486X
• DOI: 10.1007/bf00436319

✦ Synopsis

An intracellular beta-hemolysin capable of lysing human, sheep and cow erythrocytes but not cells from some other animals was isolated from the cell wails of the three developmental cell-forms of Nocardia asteroides and characterised. The spherical cell-forms contained the highest amounts of the hemolysin (100 h.u./mg protein) and the least LDs0 for mice suggesting that this may be the cell-form most pathogenic to susceptible animals. The hemolysin has the properties of a protein, was pH stable and sensitive to both catabolite repression and temperature. The activity of the hemolysin was enhanced by Ca-- § and Na + ions. The hemolysin was immunogenic in rabbits.