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Isolation and sequence analysis of the small subunit ribosomal RNA gene from the euryhaline yeastDebaryomyces hansenii

✍ Scribed by Nadathur S. Govind; Kenneth L. McNally; Robert K. Trench

Publisher
Springer-Verlag
Year
1992
Tongue
English
Weight
475 KB
Volume
22
Category
Article
ISSN
0172-8083

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✦ Synopsis

The small subunit ribosomal RNA gene (SSU rDNA) from the euryhaline yeast Debaryomyces hansenii has been isolated and sequenced. After appropriate alignment of this sequence with SSU rDNA sequences from 30 other taxa, phylogenetic reconstruction using distance matrix and maximum parsimony methods indicates that D. hansenii is most closely affiliated with Candida albicans, and occurs in the cluster of the yeasts Saccharomyces cerevisiae, Torulaspora delbruekii, Candida glabrata, and Kluyveromyces lactis. It appears that the capacity to tolerate high salt is independent of phylogenetic affiliations based on SSU rDNA analyses.

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