Isolation and quantitation of picomole quantities of digoxin, digitoxin and their metabolites by high-pressure liquid chromatography

✍ Scribed by M.C. Castle

• Book ID: 104147237
• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 591 KB
• Volume: 115
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)98946-0

✦ Synopsis

The foilowing high-pressure liquid cbromatographic (HPLC) separations are described: (I) isocratic separation of digoxin and its metabofites, (2) isocratic separation of digitoti znci its metabolites, (3) gndient eMion separation of digoxin, digitoxin and their metaboIitesf and (4) gradient ektion separation of gitoxin from digoxirr and its mefabotites. These methods utiEze a multi-wavefength =Jv detector set at 220 am and a reversed-phase co1nm.n wfth various mixtures of acetonit&e and xater as the mabile phase. The feasibiEty of using these HPLC methods as qualitative and quantitative techniques for digitalis grycosides is discussed. Digoxti and digitoxin (Fig. Ej are steroid glycosides which are widely used in the treztme=t of congestive heart failure. Ntrmerous studies have shown that these &cosides are metabolized by stepwise cleavage of the three digitoxase sugars attached at the C-3 atom of the steroid zlucleus LZ The metabolic products formed . by this cleavage process are aff czdioactive metabolites2 and ia some czses these DEITOXIS iXiOXlN GXOXW H 1 OY K xoxos~:, t.g.l.~tructures ofc3riii3c gIicosid+s.