✦ LIBER ✦
Isolation and purification oil glucuronides with preparative anion exchange chromatography in methanol
✍ Scribed by Delaborde, S. ;Loosli, H.-R. ;Ponelle, M. ;Griesser, R. ;Maurer, G.
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 491 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
890 ml of urine of rats treated with ['4C]205-734 was desalted over an Amberlite XAD-2 adsorption column. The methanol extract dissolved in 10 ml methanol was injected onto an anion exchangecolumn (Nucleosil 10 SB 250 X 1 Omm i.d.) equilibrated in methanol. A gradient of decreasing methanol proportion ver- sus a 0.2 u ammonium hydrogen carbonate buffer eluted a pure glucuronide of 205-734. Its structure was established by NMR, MS, UV, and IRanalyses. This procedure is simple, rapid, andvery selective for the compound of interest.