✦ LIBER ✦

Isolation and purification of normal and malignant colonic plasma membranes

✍ Scribed by R. J. Jackson; H. B. Stewart; G. Sachs

Publisher: John Wiley and Sons
Year: 1977
Tongue: English
Weight: 688 KB
Volume: 40
Category: Article
ISSN: 0008-543X
DOI: 10.1002/1097-0142(197711)40:5+<2487::aid-cncr2820400915>3.0.co;2-u

No coin nor oath required. For personal study only.

✦ Synopsis

Plasma membrane fractions from normal colon cells and a transplantable colon adenocarcinoma were isolated and purified by differential and zonal density centrifugation. Enrichment of normal and adenocarcinoma plasma membranes was found in zonal fractions I and I1 (ZI and ZII) following centrifugation in an 18-50'70 sucrose gradient. The distribution of various marker enzymes in normal colon preparations suggested an apical origin for the membranes obtained in zonal fraction I while zonal fraction I1 appeared to contain basal-lateral membrane fragments. Enzymatic analysis of the plasma membranes derived from the colon tumor indicated that these fractions possess a more uniform distribution of Na-K+ ATPase perhaps reflecting a dedifferentiated state. The plasma membrane fractions isolated should prove useful for investigation of transport and other properties of vesicles derived from malignant and normal colon cells.

📜 SIMILAR VOLUMES

Purification and characterization of pla

Purification and characterization of plasma membranes obtained from rat neurons prepared by bulk-isolation

✍ Dr. S. E. Poduslo; T. Chechik; M. T. Filbin; J. Rosenfeld 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 872 KB

The properties of neuronal plasma membranes are highly specialized, since they include the receptors by which many hormones, neurotransmitters, drugs, and toxins initiate their actions, as well as the components necessary for receiving synaptic input from a variety of sources and for generating the

Isolation and characterization of membra

Isolation and characterization of membrane-associated proteoglycans from normal and malignant human mammary epithelial cells

✍ D Channe Gowda; V P Bhavanandan; Eugene A Davidson 📂 Article 📅 1986 🏛 Springer US 🌐 English ⚖ 980 KB

Survival and Proliferation Factors of No

Survival and Proliferation Factors of Normal and Malignant Plasma Cells

✍ Bernard Klein; Karin Tarte; Michel Jourdan; Karene Mathouk; Jerome Moreaux; Eric 📂 Article 📅 2003 🏛 Carden Jennings Publishing 🌐 English ⚖ 243 KB

Large seale isolation and storage of Neu

Large seale isolation and storage of Neurospora plasma membranes

✍ Paul Stroobant; Gene A. Scarborough 📂 Article 📅 1979 🏛 Elsevier Science 🌐 English ⚖ 428 KB

Lipid composition of the plasma membrane

Lipid composition of the plasma membrane isolated from normal and precancerous rat bladder epithelium

✍ Mimata, H. ;Ogata, J. ;Takeshita, M. ;Shimada, T. 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 493 KB

Four-week-old Wistar strain rats were given 0.05% N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) solution for 12 weeks and used as a precancerous model. Sixteen-week-old rats of the same strain were used as controls. Plasma membranes (PMs) were prepared from normal and precancerous bladder epithelium.

Isolation and Lipid Composition of Apica

Isolation and Lipid Composition of Apical and Basolateral Membranes of Colonic Segments of Guinea Pig

✍ H.-D. Meyer zu Düttingdorf; H.-P. Sallmann; U. Glockenthör; W. von Engelhardt; R 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 128 KB

In adapting several methods of membrane isolation we established a successful way to purify apical and basolateral membranes of guinea pig colon in a parallel procedure. The conventional purification control by marker enzymes was applied. In addition, luminal membrane proteins were stained with Texa