Isolation and properties of temperature-sensitive mutants of the trfA gene of the broad host range plasmid RK2
✍ Scribed by Svein Valla; Kåre Haugan; Ross Durland; Donald R. Helinski
- Book ID
- 119079060
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 551 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0147-619X
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📜 SIMILAR VOLUMES
Transcription at various points in the trf A region of broad host range plasmid RK2 has been analysed by measuring expression of the galK gene inserted at EcoRI sites introduced previously by TB1723 transposition mutagenesis. Rightward transcription (anti-clockwise on RK2) probably from a single pro
Figurski et al. (1982) have reported that certain loci on the broad host range plasmid RK2 (kil functions) can be cloned only in the presence of other trans-acting segments of the plasmid genome (kor functions). They have suggested that the presence of these functions may in part account for the str