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Isolation and properties of protoplasts from leaves of healthy and viroid-infected tomato plants

✍ Scribed by Mühlbach, H.P.; Camacho-Henriquez, A.; Sänger, H.L.

Book ID
122549391
Publisher
Elsevier Science
Year
1977
Weight
418 KB
Volume
8
Category
Article
ISSN
0304-4211

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Yields of 106-108 peach mesophyll cells and protoplasts-gfw -1 were obtained depending on factors such as digesting enzymes, and leaf size. Onozuka R-10 (2%) in combination with Macerase (0.5%) was found best for protoplast isolation and mediocre for cell isolation among several enzyme combinations