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Isolation and Properties of Isoleucyl-tRNA Synthetase from Escherichia coli MRE 600

✍ Scribed by Andrija DurekoviĆ; Josef Flossdorf; Maria-Regina Kula


Book ID
115113710
Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
518 KB
Volume
36
Category
Article
ISSN
1432-1327

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✍ Stefica Granda; Helmut Hustedt; Josef Flossdorf; Maria-Regina Kula 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 513 KB

A procedure for the large-scale isolation of leucyl-tRNA synthetase from E. cole MRE 600 is described: The enzyme was purified about 320-fold to homogeneity by precipitation with cetyl-trimethyl-ammonium bromide, two consecutive chromatographies on DEAE-cellulose and three on hydroxyapatite with an