Isolation and binding properties of leuc
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Stefica Granda; Helmut Hustedt; Josef Flossdorf; Maria-Regina Kula
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Article
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1979
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Springer
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English
⚖ 513 KB
A procedure for the large-scale isolation of leucyl-tRNA synthetase from E. cole MRE 600 is described: The enzyme was purified about 320-fold to homogeneity by precipitation with cetyl-trimethyl-ammonium bromide, two consecutive chromatographies on DEAE-cellulose and three on hydroxyapatite with an