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Isolation and properties of a rapidly renaturing fraction of mammalian DNA

✍ Scribed by M. Brahic; M.J. Fraser


Book ID
115744362
Publisher
Elsevier Science
Year
1971
Weight
908 KB
Volume
240
Category
Article
ISSN
0005-2787

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The DNA coding for 28 S and 18 S ribosomal RNA, including the spacer regions, has been isolated from calf (Bos taurus) thymus gland. The method used included shearing of the total DNA to a highly homogeneous size population, selective heat denaturation and S1 nuclease treatment to remove single stra