Isolation and physical characterization of plasmid pCCl fromCorynebacterium callunaeand construction of hybrid derivatives

✍ Scribed by H. Sandoval; A. Aguilar; C. Paniagua; J. F. Martín

• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 654 KB
• Volume: 19
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00454379

✦ Synopsis

The corynebacteria seem to be the most suitable microorganisms for cloning genes involved in the production of amino acids, nucleotides, and other products of industrial interest. A plasmid, pCC1, from Corynebacterium callunae has been found with a size of 4.3 kb. A physical map obtained with restriction endonucleases is presented, pCC1 has single restriction sites for Kpn I, Sma I, Bal I, and Hind III. Copy number of this plasmid has been estimated to be about 30. A number of hybrid plasmids have been constructed between pCC1 and pBR329 from Escherichia coli and transformed into corynebacteria. The thiostrepton resistance gene (tsr) from Streptomyces azureus has been inserted into them.