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Isolation and partial characterization of plasmid DNA fromArthrobacter oxidans

โœ Scribed by Roderich Brandsch; Karl Decker

Publisher
Springer
Year
1984
Tongue
English
Weight
527 KB
Volume
138
Category
Article
ISSN
0302-8933

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โœฆ Synopsis

A method for the extraction of the high molecular weight plasmid AO 1 from the gram-positive soil bacterium Arthrobacter oxidans is presented.

Following digestion of this DNA with the restriction endonucleases AccI, Bam HI, Eco RI and Hind III, an average molecular mass of 157.8 kb was estimated. This value is in good agreement with the 160 kb size determined previously by electron microscopy (Brandsch et al. 1982).

Using the same method, no plasmid DNA was found in strains of the genus Arthrobacter which do not degrade nicotine, e.g., A. albidus, A. globiformis and A. auricans.

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