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Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase

✍ Scribed by Miguel A. Blázquez; Elisa Santos; Carmen-lisset Flores; José M. Martínez-Zapater; Julio Salinas; Carlos Gancedo

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
557 KB
Volume
13
Category
Article
ISSN
0960-7412

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✦ Synopsis

Summary

An Arabidopsis thaliana cDNA clone, AtTPS1, that encodes a trehalose‐6‐phosphate synthase was isolated. The identity of this protein is supported by both structural and functional evidence. On one hand, the predicted sequence of the protein encoded by AtTPS1 showed a high degree of similarity with trehalose‐6‐phosphate synthases of different organisms. On the other hand, expression of the AtTPS1 cDNA in the yeast tps1 mutant restored its ability to synthesize trehalose and suppressed its growth defect related to the lack of trehalose‐6‐phosphate. Genomic organization and expression analyses suggest that AtTPS1 is a single‐copy gene and is expressed constitutively at very low levels.

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In many organisms, trehalose protects against several environmental stresses, such as heat, desiccation, and salt, probably by stabilizing protein structures and lipid membranes. Trehalose synthesis in yeast is mediated by a complex of trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate