Isolation and mapping of a uracil-sensitive mutant of Salmonella typhimurium
β Scribed by Bussey, Lee B. ;Ingraham, John L.
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 191 KB
- Volume
- 185
- Category
- Article
- ISSN
- 0026-8925
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We have designed a new medium for the differentiation of mutants of Salmonella typhimurium defective in the ability to reduce nitrate with formate, and have characterized 24 formate dehydrogenase (FDH) mutants isolated on this medium. The mutants were assayed for the ability to use formate to reduce
## Abstract __Salmonella typhimurium__ strain 4a is a temperatureβsensitive division mutant. At 39Β° or 42Β° in several media a septation lesion occurs since nonβseptate filaments form at these temperatures. At lower temperatures in some media separation is aberrant since long chains of cells are for