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Isolation and identification of theAspergillus nidulans gdhAgene encoding NADP-linked glutamate dehydrogenase

✍ Scribed by Sarah Jane Guff; Alastair R. Hawkins; Constantin Drainas; James R. Kinghorn

Publisher: Springer-Verlag
Year: 1986
Tongue: English
Weight: 493 KB
Volume: 10
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00405099

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✦ Synopsis

The Neurospora crassa am gene was used as a heterologous probe to identify clones from two independently constructed Aspergillus nidulans gene libraries. These clones have a common HindlII 1.85 kb fragment. This A. nidulans nucleotide stretch hybridises to a N. crassa 2.7 kb BamHI fragment of wild type DNA but not to a co-migrating fragment from the DNA of the N. crassa am132 deletion mutant. One A. nidulans clone was shown to complement the N. crasse am132 deletion strain. TheN. crassa transformants show low levels (~5%) of heterologous glutamate dehydrogenase activity. The A. nidulans gdhA gene was found to locate in N. crassa at both the "homologous" (i.e. am) site as well as nonnomologous sites. Partial nucleotide analysis of the fragment has revealed the 5' end of the locus and considerable homology with the N. crassa am gene. We concluded that we have cloned the A. nidulans gdhA gene.

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✍ Hawkins, Alastair R. ;Gurr, Sarah Jane ;Montague, Paul ;Kinghorn, James R. 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 765 KB

The nucleotide sequence of the Aspergillus nidulans gdhA gene encoding NADP linked glutamate dehydrogenase has been determined and Northern blot analysis used to study the regulation of expression of this gene. The gdhA gene is 1485 nucleotides long and, by comparison with the corresponding Neurospo