Isolation and identification of enteroviruses from faecal samples in a differentiated epithelial cell line (HRT-18) derived from human rectal carcinoma
✍ Scribed by J. R. Patel; J. Daniel; M. Mathan; V. I. Mathan
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 459 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
A human rectal carcinoma-derived differentiated epithelial cell line, HRT-18, was inoculated with faecal samples in an attempt to grow coronavirus-like particles (CVLP), which are widely prevalent in human stools in southern India. While CVLP did not grow in this cell line, a variety of enteroviruses were isolated from 48 of the 114 stool samples from healthy controls and patients with diarrhoea. The results suggest that human gastrointestinal tumour-derived differentiated epithelial cells in continuous culture may be useful for the primary isolation of enteroviruses and merit further study.