Isolation and identification of 26-hydroxyecdysone 2-phosphate: An ecdysteroid conjugate of eggs and ovaries of the tobacco hornworm, Manduca sexta

✍ Scribed by Malcolm J. Thompson; James A. Svoboda; Huw H. Rees; Kenneth R. Wilzer

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 642 KB
• Volume: 4
• Category: Article
• ISSN: 0739-4462
• DOI: 10.1002/arch.940040304

✦ Synopsis

Maturing eggs (48 to 64 h old) of the tobacco hornworm, Manduca sexta, contain at least three ecdysteroid conjugates, two of which have been previously identified as 26-hydroxyecdysone 26-phosphate (the major conjugate) and 26-hydroxyecdysone 22-glucoside. In this study we have isolated and identified the third conjugate as 2dhydroxyecdysone 2phosphate by XAD-2 chromatography, C18 SEP-PAK separation, ion suppression reversed-phase high-performance liquid chromatography, nuclear magnetic resonance, and fast atom bombardment mass spectrometry. This compound is the second most abundant conjugate of ovaries from 4-day-old adult females. The possible role for this ecdysteroid conjugate is discussed.