Isolation of protoplasts and vacuoles fr
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Elisabeth Häusler; Maike Petersen; August Wilhelm Alfermann
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Article
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1993
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Springer
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English
⚖ 326 KB
In order to study the accumulation and transport of rosmarinic acid in suspension cells of Coleus blumei we established an efficient method to isolate protoplasts and vacuoles. Protoplasts were disrupted by an osmotic shock in a medium with basic pH containing ethylenediamine tetraacetic acid. The r