𝔖 Bobbio Scriptorium
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Isolation and growth of protoplasts from cell suspensions ofPinus contortadougl. ex loud

✍ Scribed by I. C. Hakman; S. Arnold


Book ID
104694696
Publisher
Springer
Year
1983
Tongue
English
Weight
598 KB
Volume
2
Category
Article
ISSN
0721-7714

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In order to study the accumulation and transport of rosmarinic acid in suspension cells of Coleus blumei we established an efficient method to isolate protoplasts and vacuoles. Protoplasts were disrupted by an osmotic shock in a medium with basic pH containing ethylenediamine tetraacetic acid. The r