Isolation and genetic study ofp-fluoro-dl-phenylalanine-resistant mutants overproducing β-phenethyl-alcohol inSaccharomyces cerevisiae
✍ Scribed by Kazuro Fukuda; Makoto Watanabe; Kozo Asano; Kozo Ouchi; Seigo Takasawa
- Publisher
- Springer-Verlag
- Year
- 1991
- Tongue
- English
- Weight
- 411 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0172-8083
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✦ Synopsis
p-Fluoro-DL-phenylalanine (PFP)-resistant mutants which produce a large amount of beta-phenethyl-alcohol, a rose-like flavor component, were isolated from the isogenic strains X2180-1A and X2180-1B of Saccharomyces cerevisiae. Cells of these mutants accumulated phenylalanine and tryptophan more than 3-fold times that of wild-type cells, while they accumulated less than half the tyrosine. The activity of prephenate dehydrogenase (PDG) (EC 1.3.1.12) was markedly decreased while that of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (EC 4.1.2.15) was increased. Genetic analysis revealed that the mutation occurred at the TYR1 locus, encoding PDG, and that the mutated TYR1 gene, try1-pfp, caused both PFP resistance and beta-phenethyl-alcohol overproduction. This was supported by molecular genetic studies with cloned tyr1-pfp DNA.