Isolation and Genetic Characterization of Streptomycin and Chloramphenicol Resistant Mutants in the Moss Physcomitrella patens
β Scribed by P. A. Collier and K. W. Hughes
- Book ID
- 125615398
- Publisher
- Society for In Vitro Biology
- Year
- 1982
- Tongue
- English
- Weight
- 886 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1475-2689
- DOI
- 10.2307/20170496
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Spontaneous mutants of the petite-negative yeast Kluyveromyces lactis, resistant to the antibiotics chloramphenicol and oligomycin, were isolated and genetically characterized. Three chloramphenicol-resistant mutants showed non-Mendelian inheritance when crossed to sensitive parents. Of 5 oligomycin
Mutants resistant to 200 micrograms/ml of the alkaloid lycorine (LYCR) in non-fermentable substrate were isolated after nitrosoguanidine mutagenesis. Tetrad analysis and growth of heterozygous (LYCR/lycS) diploids from two different mutants revealed that a single nuclear and dominant mutation is res