Isolation and expression of the gene encoding mitochondrial ADP/ATP carrier (AAC) from the pathogenic yeastCandida parapsilosis
✍ Scribed by Neboháčová, Martina; Mentel, Marek; Nosek, Jozef; Kolarov, Jordan
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 280 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0749-503X
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✦ Synopsis
A gene homologous to Saccharomyces cerevisiae AAC genes coding for mitochondrial ADP/ATP carriers has been cloned from the pathogenic yeast Candida parapsilosis. A probe obtained by PCR amplification from C. parapsilosis DNA, using primers derived from the conserved transmembrane region of yeast ADP/ATP carriers, was used for screening of the C. parapsilosis genomic library. The cloned gene was sequenced and found to encode a polypeptide of 303 amino acids that shows homology with other yeast and fungal mitochondrial ADP/ATP carriers. The gene was designated CpAAC1 and was able to complement the growth phenotypes of S. cerevisiae double deletion mutant ( aac2; aac3). The expression of the CpAAC1 gene was reduced under semi-anaerobic conditions and it was affected at normal aerobic conditions by the nature of carbon sources used for growth. Hybridization experiments indicate that C. parapsilosis possesses a single gene encoding a mitochondrial ADP/ATP carrier.