✦ LIBER ✦

Isolation and expression of a Bacillus cereus gene encoding benzil reductase

✍ Scribed by Reiji Maruyama; Mikio Nishizawa; Yasushi Itoi; Seiji Ito; Masami Inoue

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 132 KB
Volume: 75
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.1191

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Benzil was reduced stereospecifically to (S)‐benzoin by Bacillus cereus strain Tim‐r01. To isolate the gene responsible for asymmetric reduction, we constructed a library consisting of Escherichia coli clones that harbored plasmids expressing Bacillus cereus genes. The library was screened using the halo formation assay, and one clone showed benzil reduction to (S)‐benzoin. Thus, this clone seemed to carry a plasmid encoding a Bacillus cereus benzil reductase. The deduced amino acid sequence had marked homologies to the Bacillus subtilis yueD protein (41% identity), the yeast open reading frame YIR036C protein (31%), and the mammalian sepiapterin reductases (28% to 30%), suggesting that benzil reductase is a novel short‐chain de‐hydrogenases/ reductase. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 630–633, 2001.

📜 SIMILAR VOLUMES

Isolation and expression of a cDNA clone

Isolation and expression of a cDNA clone encoding HLA-Cw6: unique characteristics of HLA-C encoded gene products

✍ Shinichi Mizuno; Soo Hyoung Kang; Han Woong Lee; Joseph A. Trapani; Bo Dupont; S 📂 Article 📅 1989 🏛 Springer-Verlag 🌐 English ⚖ 710 KB

Isolation and expression analysis of two

Isolation and expression analysis of two rice genes encoding the major intrinsic protein

✍ Qiang Liu; Masaaki Umeda; Hirofumi Uchimiya 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 855 KB

We isolated two rice cDNAs (rMipl and rTipl) which are homologous to the genes encoding the major intrinsic protein (Mip) (soybean nod-26 and Arabidopsis y-Tip), respectively. Expression of rTipl in shoots and roots of rice seedlings was enhanced by water stress, salt stress and exogenous ABA. rMipl

Isolation and characterization of a cDNA

Isolation and characterization of a cDNA fromCuphea lanceolata encoding a β-ketoacyl-ACP reductase

✍ Klein, Barbara ;Pawlowski, Katharina ;Höricke-Grandpierre, Christa ;Schell, Jeff 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 879 KB

Use of an operon fusion to induce expres

Use of an operon fusion to induce expression and crystallisation of a Bacillus thuringiensis δ-endotoxin encoded by a cryptic gene

✍ Crickmore, Neil ;Wheeler, Vanessa C. ;Ellar, David J. 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 586 KB

Cloning and expression of a Bacillus cel

Cloning and expression of a Bacillus cellulase gene in Escherichia coli

✍ Seung Hwan Park; M.Y. Pack 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 434 KB

Structure and expression of a sugarcane

Structure and expression of a sugarcane gene encoding a housekeeping phosphoenolpyruvate carboxylase

✍ Henrik A. Albert; Thomas Martin; Samuel S. M. Sun 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 731 KB

A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca. 6800 bp long, with 10 exons. The entire gene sequence from -1561 to 262 bp downstream of the putative poly(A) addition signal is reported. A