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Isolation and enzymatic characterization of the plasmalemma from bovine spermatozoa

✍ Scribed by Casali, E. ;Farruggia, G. ;Spisni, A. ;Pasquali-Ronchetti, I. ;Masotti, L.

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 393 KB
Volume: 235
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402350311

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✦ Synopsis

An improved method for the isolation of pure plasma and acrosomal membranes from bull spermatozoa is presented. Plasma membranes were isolated from the spermatozoa of bulls of different breeds, and some enzymatic activity, such as (Na+-K+) ATPase, Ca++ ATPase, Mg++ ATPase, alkaline and acidic phosphatases were assayed. Such enzymatic activity levels differ noticeably from those published by other authors, whose preparations were probably contaminated by other cellular components. Highly statistically significant differences of these activities have been found among the several breeds.

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