Plating of isolated tobacco mesophyll pr
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Toshiyuki Nagata; Itaru Takebe
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Article
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1971
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Springer-Verlag
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English
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A technique was developed to derive cell and plant clones from isolated mesophyll protoplasts of tobacco. The protoplasts, plated on a fully defined agar medium, divided and grew actively forming visible colonies after one month of culture. Efficiency of colony formation depended on cell density and