Isolation and culture of suspension-derived protoplasts ofBeta vulgarisL.
β Scribed by A. Majewska-Sawka; H. Nakashima; K. Mori
- Publisher
- Springer Netherlands
- Year
- 1994
- Tongue
- English
- Weight
- 760 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0006-3134
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In order to study the accumulation and transport of rosmarinic acid in suspension cells of Coleus blumei we established an efficient method to isolate protoplasts and vacuoles. Protoplasts were disrupted by an osmotic shock in a medium with basic pH containing ethylenediamine tetraacetic acid. The r
Protoplasts were isolated from fast-growing embryogenic suspension cultures of red fescue cv. Dawson (Festuca rubra L.) without agitation. The enzyme isolation solution was highly efficient at releasing protoplasts of greater than 95% viability (5Γ10(6)-10(7) protoplasts per ml of packed cell volume