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Isolation and culture of mesophyll protoplasts ofHyoscyamus nigerL. var.annuussims

✍ Scribed by H. W. Kohlenbach; E. Bohnke


Book ID
112657829
Publisher
Springer
Year
1975
Tongue
English
Weight
990 KB
Volume
31
Category
Article
ISSN
1420-682X

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Isolation of biochemical mutants using h
✍ AndrΓ© Strauss; Franziska Bucher; Patrick J. King πŸ“‚ Article πŸ“… 1981 πŸ› Springer-Verlag 🌐 English βš– 518 KB

A population of 3070 clones derived from N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-treated mesophyll protoplasts of haploid Hyoscyamus muticus was tested for amino-acid auxotrophy without enrichment. One clone (MA-2) was stably and specifically dependent on casein hydrolysate and could be fed also