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Isolation and confirmation of function of the Coccidioides immitis URA5 (orotate phosphoribosyl transferase) gene

✍ Scribed by Jieh-Juen Yu; Li Zheng; Pei W. Thomas; Paul J. Szaniszlo; Garry T. Cole


Book ID
117523441
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
694 KB
Volume
226
Category
Article
ISSN
0378-1119

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TheURA5 gene encoding orotate-phosphorib
✍ Bai, Xiyuan; Larsen, Michael; Meinhardt, Friedhelm πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 264 KB πŸ‘ 2 views

A pair of degenerate primers was used for amplification and cloning of an internal fragment of the K. lactis URA5 gene. Primers were designed on the basis of highly conserved motifs within protein sequences predicted for URA5 genes from several microorganisms. Using the amplified fragment as a probe