## To evaluate CENP-A reactivity with anticentromere antibodies (ACA) using recombinant protein (rCENP-A). Methods. Human CENP-A antigen was overexpressed in insect cells using the baculovirus system. We tested for ACA activity against the full-length recombinant polypeptide by immunoblot and by e
Isolation and Comparison of Natural and Recombinant Human CENP-A Autoantigen
β Scribed by Antigona Martinez; Dongxu Sun; Peter B Billings; Kristine M Swiderek; Kevin F Sullivan; Sallie O Hoch
- Book ID
- 115621614
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 248 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0896-8411
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p330d, also known as CENP-F, is a newly characterized cell cycle specific nuclear autoantigen which is associated both with the centromeres and the nuclear matrix. It is expressed in low amounts in GO/Gl-cells and accumulates in the nuclear matrix during S-phase with a maximum expression in G2/M-cel
## Protein blot assays specific for the discrimination of the centromere autoantigen, CENP-A, from human cells The Agouron Institute, La Jolla, CA The Western or protein blot has proven to be a valuable resource in detecting discrete, immunoreactive antigen targets associated with a variety of au