Isolation and characterization of α-tubulin genes fromSeptoria tritici andRhynchosporium secalis, and comparative analysis of fungal α-tubulin sequences
✍ Scribed by Rohel, Eric A. ;Payne, Andrew C. ;Hall, Len ;Barker, Helen ;Butters, Jenny ;Hollomon, Derek W.
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 159 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0886-1544
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✦ Synopsis
The ␣-tubulin genes from Septoria tritici and Rhynchosporium secalis have been cloned and sequenced. The predicted amino acid sequence and intron structure showed strong homology with other known filamentous fungal ␣-tubulins. Comparison of sixteen fungal ␣-tubulin sequences based on amino acid sequence homology and intron structure identified five groups of proteins. Group 1 consists of filamentous fungi, including S. tritici and R. secalis, the dimorphic fungus Histoplasma capsulatum, and Pneumocystis carinii. Group 2 includes two divergent isoforms from Neurospora crassa and Aspergillus nidulans. Group 3 includes the yeast Saccharomyces cerevisiae and the dimorphic fungus Candida albicans. Group 4 contains the single yeast Schizosaccharomyces pombe. Group 5 includes the only Basidiomycete, Schizophyllum commune. This analysis supports the classification of P. carinii as a primitive Ascomycete. The presence of an additional glycine residue between the second and third amino acid found only in Group 2 proteins may indicate a functionally distinct fungal isotype. Implications in terms of structure-function relationships for ␣-tubulin molecules are discussed.