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Isolation and characterization of two distinct cDNA clones encoding corn seed cysteine proteinases

✍ Scribed by Chieko Domoto; Hirohito Watanabe; Makoto Abe; Keiko Abe; Soichi Arai

Book ID
113263412
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
435 KB
Volume
1263
Category
Article
ISSN
0167-4781

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PCR cloning and expression analysis of c
PCR cloning and expression analysis of cDNAs encoding cysteine proteinases from germinating seeds ofVicia sativaL.
✍ Claudia Becker; JΓΌrgen Fischer; Van Hai Nong; Klaus MΓΌnitz πŸ“‚ Article πŸ“… 1994 πŸ› Springer 🌐 English βš– 756 KB

cDNA clones encoding cysteine proteinases from cotyledons of germinated seeds of Vicia sativa L. have been obtained by means of PCR. Degenerate oligonucleotide primers were designed according to conserved amino acid regions of known cysteine proteinases. The deduced amino acid sequences of the cDNA