Isolation and characterization of tracheobronchial mucin from a laryngectomee

✍ Scribed by Lawrence A. Tabak; Molakala S. Reddy; Laura D. Monte; Michael J. Levine

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 825 KB
• Volume: 135
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(84)85009-0

✦ Synopsis

A tracheobronchial mucin was isolated from the tracheobronchial secretion of a laryngectomee.

It was purified by gel filtration on Sepharose CL-6B in Trisurea buffer and rechromatography of excluded materials through the same gel matrix. It was homogeneous in 0.7% agarose-2% polyacrylamide electrophoresis under nonreducing conditions. Comparable analysis with 2-mercaptoethanol revealed at least 3 subunits. Based upon recoverable weight, the mucin was composed of 75% carbohydrate, 21% protein, and 3% sulfate. Oligosaccharides obtained by alkaline p-elimination indicated 0-glycosyl linkage to the peptide component. Marked heterogeneity of the carbohydrate side-chains was reflected in the preparation of 20 distinct oligosaccharides ranging in size from 4 to 17 residues.