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Isolation and characterization of the Schizosaccharomyces pombe gene encoding transcript elongation factor TFIIS

✍ Scribed by Williams, Laura A.; Kane, Caroline M.

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
992 KB
Volume
12
Category
Article
ISSN
0749-503X

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✦ Synopsis

A gene designated tfsl has been isolated from Schizosaccharomyces pombe based on its similarity to genes encoding transcription elongation factor TFIIS. The nucleotide sequence of the tfsl gene predicts a polypeptide with similarity to mammalian, Drosophila and Saccharomyces cerevisiae TFIIS. A haploid Sz. pombe strain with tfsl deleted from the genome is viable. Thus, tfsl is not essential for viability. However, deletion of tfil results in slow growth and increased sensitivity to the drug 6-azauracil, a phenotype similar to that of a S. cerevisiae strain deleted for the gene encoding TFIIS. The DNA sequence of tfsl has been deposited in GenBank under Accession Number U20526.

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