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Isolation and characterization of the hisG and hisD genes of Klebsiella pneumoniae

โœ Scribed by Rodriguez, Raymond L.; West, Robert W.; Tait, Robert C.; Jaynes, Jesse M.; Shanmugam, K.T.

Book ID
122373371
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
376 KB
Volume
16
Category
Article
ISSN
0378-1119

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From a gene bank of Klebsiella pneumoniae M5a1, a 1.7 kb gene fragment was isolated which was able to restore the Ntr+ phenotype and ammonium (methylammonium) transport, but not glutamate synthase in an Escherichia coli glt mutant (glutamate synthase deficiency). The fragment strongly hybridized wit