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Isolation and characterization of the gene encoding phosphoenolpyruvate carboxykinase from Saccharomyces cerevisiae

✍ Scribed by Marta D. Valdés-Hevia; Raquel de la Guerra; Carlos Gancedo

Book ID
115922741
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
693 KB
Volume
258
Category
Article
ISSN
0014-5793

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Isolation and nucleotide sequence of the
Isolation and nucleotide sequence of the gene encoding phosphoenolpyruvate carboxykinase fromKluyveromyces lactis
✍ Kitamoto, Hiroko K.; Ohmomo, Sadahiro; Iimura, Yuzuru 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 148 KB 👁 2 views

The KlPCK1 gene encoding phosphoenolpyruvate carboxykinase (PEPCK; ATP-dependent) was cloned from the Kluyveromyces lactis genome using a PCR amplicon from Saccharomyces cerevisiae PCK1 gene as a probe. A DNA fragment of about 4•8 kb containing KlPCK1 complemented PEPCK activity of the mutant of S.