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Isolation and characterization of proteoglycan derived from human placenta and its biological activities

✍ Scribed by Kyung Bok Lee; Jong Sig Kim; Yung Choon Yoo; Sang Tae Kwak; Kyung Sik Song; Yeong Shik Kim


Publisher
Springer
Year
2000
Tongue
English
Weight
394 KB
Volume
23
Category
Article
ISSN
0253-6269

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Transfection of Chinese hamster ovary (CHO) cells with a plasmid containing the cDNA for human preprorenin has provided cell lines that secrete 15 mg of native prorenin per liter of culture medium. Tryptic activation of the prorenin occurs by selective cleavage of the Arg66-Leu67 bond (numbering as