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Isolation and characterization of plasma membranes from Krebs II ascite cells using percoll gradient

✍ Scribed by Michel Record; Jean-Claude Bes; Hugues Chap; Louis Douste-Blazy


Book ID
115725917
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
832 KB
Volume
688
Category
Article
ISSN
0005-2736

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The characterization of free, cytoskelet
✍ Anni Vedeler; Ian F. Pryme; John E. Hesketh πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 755 KB

Polysomes from Krebs II ascites and 3T3 cells were separated into three populations by using a sequential extraction method. Free polysomes were released by using a combination of low salt (25 mM KCl) and NP-40 detergent in the lysis buffer. The cytoskeletal bound polysomes were subsequently release