Abstract
Neuropeptides are supposed to be implicated in the regulation of hormone as well as nonhormone dependent behavioral processes in birds. Previous immunohistochemical studies have suggested that in birds opioid pentapeptides, Met‐ and Leu‐enkephalins, may be present in the brain including the regions that regulate sex hormone dependent behaviors, such as reproductive behaviors. To determine biochemically the presence of opioid peptides in the avian brain, a study was conducted to isolate these peptides from Japanese quails and zebra finches. Acetic acid extracts of the quail and finch brains were respectively forced through disposable C‐18 reversed‐phase cartridges, and then the retained material was subjected to the cation‐exchange and reversed‐phase high performance liquid chromatographic (HPLC) purifications. All of the purified substances showed a single peak on the reversed‐phase HPLC and these substances enhanced spontaneous contractions of the avian rectum. The purified bioactive substances were further subjected to amino acid sequence analysis and were characterized as peptides with the following sequences: Tyr‐Gly‐Gly‐Phe‐Met, Tyr‐Gly‐Gly‐Phe‐Leu, and Tyr‐Gly‐Gly‐Phe‐Met‐Arg‐Phe. These three peptides were identical with opioid pentapeptides, Met‐ and Leu‐enkephalins, and a heptapeptide, Met‐enkephalin‐Arg^6^‐Phe^7^, which had been previously isolated from mammalian species. This is the first direct demonstration of the presence of opioid peptides in the avian brain and confirms previous immunohistochemical findings suggesting a functional role for the opioid peptide in neural mechanisms of avian reproductive behavior. © 1995 Wiley‐Liss, Inc.